Use of the Escape nitinol stone retrieval basket facilitates
Background and purpose: Advances in ureteroscope and stone basket design have catapulted ureteroscopy to the forefront of surgical stone management; however, persistent problems such as stone migration continue to challenge urologists. The Escape nitinol stone retrieval basket (Boston Scientific, Natick, MA) is a stone basket designed to capture …
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Extraction d'ADN, PCR et séquençage de l'ADN
6. Extraction au chloroforme ou à l'éther : cette étape complète toujours une extraction phénolique, car elle permet d'éliminer les traces de phénol qui aurait pu être importé par la phase aqueuse (le phénol peut inhiber les enzymes utilisées dans l'amplification, la restriction…etc.). 7.
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At the dawn: cell-free DNA fragmentomics and gene regulation
In 2003, Deligezer et al. suggested a significant correlation between the fragmentation status in cfDNA and DNA methylation, as well as the nucleosomes, at the 1st exon of the p16 gene in lymphoma ...
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Golgi organization is regulated by proteasomal degradation
Golgi fragmentation has been described under different stress conditions and during apoptosis 62,63,64,65 and is correlated with pathology 9,10. Indeed, our observations indicate that perturbing ...
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Rock fragmentation variations with increasing extraction …
These oversize fragments are commonly observed at drawpoints in caving methods. 12 Manzoor et al. 13 documented the occurrence of oversize fragments (fragments bigger than 1 × 1 × 1 m) in more ...
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Ancient DNA Damage
Fragmentation and deamination. (A) A likely cause of fragmentation in ancient DNA is depurination, in which the N-glycosyl bond between a sugar and an adenine or guanine residue is cleaved, resulting in an abasic site.The DNA strand is then fragmented through β elimination, leaving 3′-aldehydic and 5′-phosphate ends. (B) Deamination of cytosine to …
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Green extraction optimization of triterpenoid glycoside-enriched
MAE closed-vessel system reported by Shen et al., extraction with 90% methanol at 70 °C for 20 min produced a higher C. asiatica triterpenoid yield in a shorter extraction time than sonication ...
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Evaluation of blast efficiency in aggregate quarries: facts and
Quarry blasts and seismicity. Blast operation creates both elastic and inelastic deformation within the nearby rock mass. A seismic event is a rapid inelastic deformation that occurred within a specified volume of rock (Ma et al. 2015).Regulatory authorities usually monitor blasts in quarries on the scheduled date of the operations by …
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The effect of sperm DNA fragmentation on miscarriage …
Data extraction was performed by two reviewers. Study quality was assessed using the Newcastle–Ottawa Scale. ... Zini et al.'s review identified seven studies, ... The negative impact of high DNA fragmentation on pregnancy can be overcome by using high-quality oocytes, as shown in a study comparing standard and donor cycles ...
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diaPASEF: parallel accumulation–serial fragmentation
diaPASEF makes use of the correlation between the ion mobility and the m/z of peptides to trap and release precursor ions in a TIMS-TOF mass spectrometer for an almost complete sampling of the ...
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Optimization of Blasting Parameters- A case study of …
The research was carried out as a practical method on Nchanga Open Pits (NOP) ore Bench to optimize the blasting design parameters that can yield the required fragmentation size thereby reducing ...
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Rock fragmentation variations with increasing extraction …
Shekhar et al. [Citation 16] suggest ... The relationship between Class 1 fragmentation and extraction ratio is not significant after extraction; the p-value is much higher than the significance level of 0.05, so the null hypothesis is valid. The data show a lot of variation as well (R 2 = 0.025). Similar behaviour can be observed for Class 2.
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Ureteroscopic Laser Lithotripsy: A Review of Dusting vs Fragmentation …
Clinical application of dusting and fragmentation with extraction. The question of which surgical technique is optimal for treating renal stones remains controversial and should be determined on a case-by-case basis. Fragmentation with extraction is appealing for both the surgeon and patient for many reasons . …
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(PDF) Rock Fragmentation by Blasting – a review
The fragmented material produced by blasting has a great influence on mining and construction in different aspects. [3] Cited from [4] that in mining and processing plants, rock fragmentation ...
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Fragmentation et extraction des reins polykystiques au …
Fragmentation et extraction des reins polykystiques au cours de la néphrectomie cœlioscopique manuellement assistée : une étude observationnelle prospective. ... Cependant l'extraction de ces reins de haut volume est un défi qui n'a pas été résolu de manière adéquate. Nous avons expérimenté une technique de …
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Cellular and Molecular Nature of Fragmentation of Human …
Embryo fragmentation represents a phenomenon generally characterized by the presence of membrane-bound extracellular cytoplasm into the perivitelline space. Recent evidence supports the cellular and molecular heterogeneity of embryo fragments. In this narrative review, we described the different embryo fragment-like cellular structures …
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Current Nucleic Acid Extraction Methods and Their …
1. Introduction. Nucleic acid extraction (NAE) is one of the most pivotal steps in molecular biology, being routinely used in many areas of the biological and medical sciences, as this procedure marks a starting point in any molecular diagnostic kit [].This crucial procedure has been known for over a century and has developed substantially …
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Glycoproteomics | Nature Reviews Methods Primers
Protein glycosylation is a process and is mediated by enzymes known as glycosyltransferases, responsible for the initiation or elongation of glycans, and oligosaccharyltransferases, responsible ...
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Magnetic-Activated Cell Sorting (MACS): A Useful Sperm …
Serum β-hCG analysis was performed 12 days after embryo transfer (ET), and clinical pregnancy was confirmed when a gestational sac with a fetal heartbeat was visible in an ultrasound scan. ... Sperm DNA fragmentation can also be induced by epididymal oxidative damage caused by an increase in reactive oxygen species (ROS). …
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L'extraction de l'or
L'extraction de l'or se fait dans des champs de minerais. Il existe trois principaux procédés pour extraire ce métal si cher et si rare : premièrement : la gravitation, deuxièmement : l'amalgamation et …
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Detection of cell-free DNA fragmentation and copy number
Detection of cell-free tumor DNA (cftDNA) in cerebrospinal fluid (CSF) has been proposed as an alternative to detection in plasma. We used shallow whole-genome sequencing (sWGS, at a coverage of < 0.4×) of cell-free DNA from the CSF of 13 patients with primary glioma to determine somatic copy number alterations and DNA fragmentation patterns.
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Ureteroscopic Laser Lithotripsy: A Review of Dusting …
Conclusions: Both dusting and fragmentation with extraction approaches to ureteroscopic stone treatment are effective. In fact, there is little evidence that one approach is better …
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Evaluating the quantity, quality and size distribution of cell …
Cell-free DNA (cfDNA) has become a comprehensive biomarker in the fields of non-invasive cancer detection and monitoring, organ transplantation, prenatal genetic testing and pathogen detection.
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Fragmentation et extraction des reins polykystiques au …
Fragmentation et extraction des reins polykystiques au cours de la néphrectomie cœlioscopique manuellement assistée : une étude observationnelle prospective ... Cependant l'extraction de ces reins de haut volume est un défi qui n'a pas été résolu de manière adéquate. Nous avons expérimenté une technique de …
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Inferring gene expression from cell-free DNA fragmentation …
Sun, K. et al. Orientation-aware plasma cell-free DNA fragmentation analysis in open chromatin regions informs tissue of origin. Genome Res. 29, 418–427 (2019).
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Frontiers | High Throughput Sequencing for the Detection …
In terms of bias introduced during fragmentation of CDNA, Tn5 and other enzyme-based CDNA fragmentation methods require a precise enzyme:DNA ratio, making method optimization less straightforward than RNA fragmentation (Hrdlickova et al., 2017). When the enzymatic fragmentation is run to completion, the proportion of smaller …
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DNA methylation analysis explores the molecular basis of
CfDNA extraction. For WGS and EM-seq library preparation, 600 μL and 2 mL cell-free plasma was used to extract cfDNA, respectively. ... Wang, J. et al. Altered cfDNA fragmentation profile in ...
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Ureteroscopic Laser Lithotripsy: A Review of Dusting vs …
fragmentation.11 Decreased stone movement and retro-pulsion may allow for more efficient ureteral stone treatment by minimizing the need to ''chase'' ureteral stones, which Table 1. Typical Laser Settings for Dusting vs Fragmentation with Extraction Dusting; HF/low energy Fragmentation with extraction; low frequency/high energy Frequency
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Unsupervised Structural Classification of Dissolved …
Dissolved organic matter (DOM) is considered an essential component of the Earth's ecological and biogeochemical processes. Structural information of DOM …
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Use of FFPE-derived DNA in next generation sequencing: DNA extraction
Use of these in next generation sequencing (NGS) is a priority. Nine formalin-fixed paraffin-embedded (FFPE) DNA extraction methods were evaluated using twelve FFPE samples of varying tissue types. Quality assessment included total yield, percent dsDNA, fragment analysis and multiplex PCR. After assessment, three tissue types from …
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